Nephrocalcinosis

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Nephrocalcinosis is a condition in which calcium levels in the kidneys are increased. Most often, the increase in renal calcium is generalized, as opposed to the localized increase observed in calcified renal infarct and caseating granulomas of renal tuberculosis. See the image below.

Presentation is primarily determined by the underlying etiology, though in many cases, the condition remains asymptomatic and is identified only as a radiologic abnormality. The physical findings are nonspecific and reflect the underlying disorders responsible.

Clinical features of hypercalcemic nephropathy may include the following:

Relative vasopressin resistance with decreased renal concentrating ability and increased free water diuresis, manifesting as polyuria and polydipsia

Renal glycosuria, reduced glucose tubular maximum, aminoaciduria, and nonglomerular proteinuria

Reversible hypertension

Renal failure, usually reversible but sometimes not

Clinical features of microscopic nephrocalcinosis (on the basis of animal studies) may include the following:

Reduced concentration capacity

Increased blood urea nitrogen (BUN)

Prolongation of nephron transit time in the distal tubule

Acute pyelonephritis or calculous ureteral obstruction with renal failure

Clinical features of macroscopic nephrocalcinosis (the form most commonly seen) may include the following:

Renal colic

Hematuria

Passage of urinary stones

Urinary tract infection

Polyuria and polydipsia

Hypertension

Proteinuria

In Dent disease, loss of low-molecular-weight proteins, hypercalciuria, and nephrolithiasis

Microscopic pyuria

Distal tubular dysfunction with a mild salt-losing defect

Proximal tubular dysfunction (unusual)

Secondary distal tubular acidosis

Renal failure

See Presentation for more detail.

Laboratory studies that may be useful include the following:

Serum calcium, phosphate, and albumin levels

Blood urea nitrogen (BUN) and serum creatinine levels

Estimated glomerular filtration rate (eGFR)

Serum potassium concentration

Urinalysis and urine culture

Assessment of 24-hour urinary excretion of calcium, oxalate, citrate, and uric acid

Urinary magnesium levels

Parathyroid hormone and thyroid-stimulating hormone levels

Imaging studies that may be considered include the following:

Radiography (eg, kidney-ureter-bladder [KUB])

Ultrasonography (more sensitive than conventional radiography)

Computed tomography (CT; more effective in detecting calcification)

Magnetic resonance imaging (MRI) offers no advantages over these modalities and is not warranted unless another compelling indication is present.

See Workup for more detail.

Pharmacologic and other nonsurgical treatments for hypercalcemia and hypercalcemic nephropathy include the following:

Adequate hydration with an isotonic sodium chloride solution (the single most effective measure for reversing hypercalcemia and protecting the kidneys)

Cinacalcet (for correction of hyperparathyroidism)

Chemotherapeutic agents (for osteolytic malignancies)

Steroids (to decrease intestinal calcium absorption and vitamin-D activity)

Hydroxychloroquine (for sarcoid granulomas)

Calcitonin or bisphosphonates (to inhibit bone resorption)

Pharmacologic and other nonsurgical treatments for macroscopic nephrocalcinosis include the following:

Thiazide diuretics (eg, hydrochlorothiazide)

Dietary salt restriction

Potassium and magnesium supplementation

Citrate supplementation (preferably as potassium citrate)

High-dose pyridoxine

Surgery options that may be considered for urinary stones causing obstruction include the following:

Percutaneous nephrolithotomy

Laser and shock wave lithotripsy

Stent placement

Open surgery (rarely necessary)

Parathyroidectomy may be considered for removal of enlarged adenomas.

See Treatment and Medication for more detail.

Nephrocalcinosis is a condition in which calcium levels in the kidneys are increased. There is predominantly interstitial deposit of calcium phosphate or calcium oxalate in the renal cortex and/or medulla. This condition can sometimes overlap with nephrolithiasis, which is characterized by intratubular deposits of calcium.  Calcium deposits can be detected (usually as an incidental finding) through a radiologic examination or via microscopic examination of the renal tissues. The term nephrocalcinosis most often applies to a generalized increase in interstitial renal calcium content, as opposed to the localized increase observed in calcified renal infarct and caseating granulomas of renal tuberculosis.{ref1-INVALID REFERENCE}

Nephrocalcinosis has a substantial overlap with hypercalcemia, nephrolithiasis, renal parenchymal damage, and reduced renal function. Therefore, rather being considered a single, distinct disease process, it should be viewed as a helpful finding for several distinct disease processes, one that demands evaluation. [1]

Microscopic nephrocalcinosis is characterized by the presence of microscopic crystalline calcium precipitates in the form of oxalate or phosphate. Patients with macroscopic nephrocalcinosis have larger areas of calcifications, which can be observed on visual or radiologic examination without further magnification.

Patients with hypercalcemia develop renal function abnormalities. Under these circumstances, the term hypercalcemic nephropathy is more appropriate than is the older term chemical nephrocalcinosis.

Calcium is a critical divalent cation that is transported, along with sodium, potassium, and water, in a complex and regulated manner along the renal tubular epithelium. The cytoplasmic concentration of calcium is tightly regulated and kept very low, being maintained by active extracellular extrusion of calcium and sequestration into the endoplasmic reticulum and mitochondria. Increased extracellular calcium leads to impairment of the calcium messenger system with gross tubular impairment.

The effects of increased calcium have been studied extensively in rats. Rats treated with vitamin D demonstrated mitochondrial swelling and loss of mitochondrial enzyme activities before calcification appeared. Parathyroid extract−induced hypercalcemia was found to cause changes in rat kidneys, predominantly affecting the distal nephron, with focal necrosis of the outer medullary collecting ducts and the ascending limb of the loop of Henle.

Hypercalcemia results in renal vasoconstriction and a reduced glomerular filtration rate. It also interferes with renal tubular functions. Impaired renal concentration ability and resistance to vasopressin are the most common defects observed with hypercalcemia. These changes may be mediated by reduced sodium transport in the loop of Henle (see the image below) and by antidiuretic hormone antagonism via calcium-sensing receptors, [2] or they may be related to medullary prostaglandin synthesis.

Maximum diluting capacity remains unimpaired. Effectively, the sum effect of this process will be a clinical picture resembling that of nephrogenic diabetes insipidus.

Renal sodium conservation is also impaired because of reduced absorption of sodium chloride in the medullary thick ascending limb and collecting tubule, although this rarely results in gross renal sodium losses. Potassium excretion is increased. Magnesium excretion is also increased, probably due to suppression of parathyroid hormone, which enhances tubular magnesium absorption. [3]

Hypercalcemia increases urinary calcium excretion by increasing the filtered load and reducing tubular absorption. Its effects on phosphate excretion are complex. In experimental animals, pure hypercalcemia reduces phosphate excretion; conversely, in certain cancers, it can be associated with increased phosphate excretion, but the latter occurrence is probably due to the presence of phosphaturic peptides (phosphatonins), which are secreted in some malignancies. [4, 5]

The effects on the acid-base balance are even more complex. Increased renal acid excretion occurs with intravenous (IV) calcium infusions, and metabolic alkalosis has frequently been reported in patients with hypercalcemia. On the other hand, parathyroid hormone decreases hydrogen ion excretion, leading to a distal type of renal tubular acidosis (RTA).

This opposing effect of hypercalcemia and parathyroid hormone has been used in the differential diagnosis of hypercalcemia, because serum bicarbonate is lower and chloride is higher when hyperparathyroidism is the cause of hypercalcemia.

Microscopic nephrocalcinosis has undergone considerable laboratory study. Although this condition theoretically occupies a stage between hypercalcemia and macroscopic nephrocalcinosis, it is difficult to demonstrate in humans, because renal biopsies are not routinely performed in the early stages of metabolic diseases known to lead to the macroscopic stage. However, some elegant human data are now available that demonstrate early stone formation, with blockage of the collecting tubes and subsequent inflammatory response. [6]

At autopsy, healthy human kidneys invariably contain microscopic deposits of calcium in the renal medulla. Microscopic nephrocalcinosis can occur without macroscopic involvement in patients with longstanding hypercalcemia from primary parathyroidism, milk-alkali syndrome, or primary hyperoxaluria.

Different patterns of microscopic nephrocalcinosis have been described. The cortical pattern has been found after parenteral calcium administration. The corticomedullary type involves calcium phosphate deposits that occur in the inner zone of the renal cortex and extend into the medulla. Precipitating factors include excess parathyroid hormone, vitamin D, acetazolamide, magnesium depletion, decreased urinary citrate, and hypothyroid state. Increased plasma calcium is not an essential prerequisite for this type of nephrocalcinosis.

The medullary pattern has been reported in hyaline droplet nephropathy resulting from inhalation of volatile hydrocarbons. The pelvic type affects renal papillae. The deposits usually are calcium phosphate, but calcium oxalate also has been implicated. The underlying mechanism appears to be either increased intestinal absorption or decreased renal excretion of calcium.

Macroscopic nephrocalcinosis refers to calcium deposition that is visible without magnification and usually is discovered by means of conventional radiography, ultrasonography, or computed tomography (CT) or at autopsy. Macroscopic nephrocalcinosis can affect either the cortex or the medulla, with the latter site being more commonly involved.

Cortical nephrocalcinosis is rare and usually occurs secondary to diffuse cortical disease injury. The calcification can be patchy or confluent. In chronic glomerulonephritis, calcium deposits are found most often in periglomerular tissue and not in the glomerulus. Nephrocalcinosis also has been reported in familial infantile nephrotic syndrome and in Alport syndrome.

Acute cortical necrosis secondary to toxemia of pregnancy, snakebite, or hemolytic-uremic syndrome can lead to patchy cortical nephrocalcinosis. Calcium deposition can start as early as 30 days after cortical necrosis. Chronic pyelonephritis and vesicoureteral reflux are also implicated. [7] Renal transplantation, primary hyperoxaluria, methoxyflurane abuse, autosomal recessive polycystic kidney disease, and benign nodular cortical nephrocalcinosis may be involved in cortical nephrocalcinosis, albeit rarely.

Medullary nephrocalcinosis assumes the form of small nodules of calcification clustered in each pyramid. Diagnosing the underlying renal disease on the basis of the appearance is difficult. Characteristic exceptions include papillary necrosis due to analgesic abuse and medullary sponge kidneys. [8] In papillary necrosis, the entire papilla may be calcified, whereas in medullary sponge kidney, there is a characteristic band of calcification in the renal pyramids.

It has been suggested that when hypercalcemia is the most important factor, the first foci of calcification develop in the renal tubular cells, whereas when hypercalciuria is the major factor, the initial foci form in the interstitium.

Intraluminal tubular calcium crystals are believed to serve as potential nidi for further buildup of calcium and other stone-forming substances, including oxalate and uric acid. Whether further growth of nephroliths occurs probably depends on a number of additional factors, such as abnormal urine composition, urine flow and volume, and the presence or absence of endogenous inhibitors of crystalline formation in the urine.

Etiologies of cortical calcium deposition in kidneys are as follows:

Etiologies of medullary calcium deposition in kidneys are as follows:

Primary hyperparathyroidism is the single most common cause of nephrocalcinosis in adults. Although nephrocalcinosis is a relatively rare complication (5%), primary hyperparathyroidism itself is relatively common, especially in the elderly.

Nephrocalcinosis is related more to the duration than to the intensity of hypercalcemia. The classic clinical findings are sometimes referred to as “(abdominal) groans, stones, and bones.” This common phrase is a reminder that patients may present with kidney stones, bone pain, osteoporosis, and pathologic fractures, all of which can result in abdominal discomfort. Rarely, hyperparathyroidism can be associated with multiple endocrine neoplasia type 1 (MEN1).

Distal RTA is the second most common cause of medullary nephrocalcinosis. The familial form and the secondary form (autoimmune-associated anti-K/H channel antibody) are common. [9] The mechanisms contributing to nephrocalcinosis in distal RTA are hypercalcemia, hypercalciuria, metabolic acidosis, and reduced citrate excretion in the presence of increased urinary pH. [10] Because medullary nephrocalcinosis itself can cause distal RTA, distinguishing the initial insult can be difficult. Renal function is fairly well maintained.

Other causes of nephrocalcinosis are hypervitaminosis-D states [11] resulting from excessive treatment of hypoparathyroidism, self-administration of vitamins, and the presence of a granulomatous disease, such as sarcoidosis. [12]  

In granulomatous disorders, conversion of 25-hydroxycholecalciferol to 1,25-dihydroxycholecalciferol in the granuloma is increased, resulting in an unregulated production of bioactive vitamin D with resultant excessive intestinal absorption of calcium and phosphorus. In addition, cytokines (eg, interleukin [IL]-2) released in these disorders cause dysregulation of calcium homeostasis and activation of osteoclasts, resulting in subacute and chronic hypercalcemia.

Any other cause of hypercalcemia, particularly when associated with hypercalciuria, can contribute to nephrocalcinosis. Etiologies include milk-alkali syndrome (due to excess ingestion of antacids), hyperparathyroidism, and malignant disease (due to bone involvement and humoral factors, including cytokines and parathyroid hormone-related peptide). Idiopathic hypercalciuria, [13, 14] a common metabolic disease, is also known to cause nephrocalcinosis.

Nephrocalcinosis and renal failure are increasingly being recognized as common complications of phosphate supplementation, particularly in the elderly. [15, 16, 17, 18, 19] Other possible risk factors are preexisting renal failure, high blood pressure, and pharmacologic treatment of high blood pressure (eg, with angiotensin-converting enzyme [ACE] inhibitors or angiotensin-receptor blockers [ARBs]).

Phosphate supplements may contribute to renal calcifications in children with hypophosphatemic rickets. In vitro studies have shown that an increased urinary concentration of phosphate can result in intratubular crystallization with altered solubility.

Medullary sponge kidney is a common cause of medullary calcification, with calcium lying in dilated collecting ducts rather than in the renal substance. These ectatic outpouchings are believed to be areas of urinary stasis possessing the ideal characteristics for fostering the formation of these calcifying complexes. The calcium deposits are larger and more sharply defined than they are in metabolic disease, and they are uneven in distribution. [8] Associated hemihypertrophy of the body may exist.

Unlike the severe renal damage with minimal calcification associated with hypercalcemic states, nephrocalcinosis associated with distal RTA and medullary sponge kidney usually is gross, and renal function is relatively well preserved.

Renal papillary necrosis associated with analgesic nephropathy is identified as calcified papillae rather than as a speckled pattern.

Other associations with nephrocalcinosis include rapidly progressive osteoporosis due to immobilization, menopause, aging, or steroids.

Medullary calcification can be induced by primary (familial) hyperoxaluria or by secondary hyperoxaluria due to increased intake of oxalates, enhanced absorption resulting from intestinal disease, or ingestion of ethylene glycol or methoxyflurane. [20, 21] Primary hyperoxaluria and ethylene glycol intoxication are also associated with diffuse calcium-oxalate depositions in many other organs, including the eye and the heart.

Chronic hypokalemic states, such as Bartter syndrome, primary hyperaldosteronism, Liddle syndrome, and 11-beta hydroxylase deficiency, are associated with reduced urine citrate excretion and tubular epithelial damage, leading to calcium precipitations.

Autosomal dominant hypophosphatemic rickets and X-linked hypophosphatemic conditions [22] have been associated with abnormal phosphate wastage and nephrocalcinosis due to elevated levels of phosphatonins (fibroblast growth factor 23; secreted frizzled-related protein 4). [4, 5] Nephrocalcinosis is very common (frequency ~80% on ultrasonography) and may be associated with phosphate supplementation for the condition.

Dent disease and familial magnesium-losing nephropathy are rare inherited diseases causing medullary calcification.

Dent disease (also referred to as X-linked recessive hypophosphatemic rickets [in Italy], X-linked recessive nephrolithiasis, and idiopathic low-molecular weight proteinuria with hypercalciuria and nephrocalcinosis [in Japan]), arises from a defect in a gene on the short arm of the X chromosome that codes for the renal chloride channel in the proximal tubule (CLC-5). Mutations in the OCRL-1 gene—normally associated with Lowe syndrome—have been described in cases of clinical Dent disease, expanding the potential for genetic diversity. [23]

Inherited forms of magnesium-losing nephropathy have been described. [24] Familial hypomagnesemia hypercalciuric nephrocalcinosis (FHHNC) is an autosomal recessive disease associated with cation loss through a defect in a renal tight junction protein claudin-16 (paracellin-1) [25, 26, 27]  or claudin-19 [28, 29] involved in paracellular transport. Claudin-16/paracellin-1 is dysfunctional in familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). However, hypomagnesemia is not a mandatory finding in all kindreds [30] . Thiazide-type diuretics appears efffective in lessning hypercelcuria and nephrocalcinosis. 

Associated malignancies are not typical in nephrocalcinosis, because patients seldom survive long enough with hypercalcemia to develop them; a possible exception is parathyroid carcinoma.

Familial benign hypercalcemia and hyperthyroidism are not associated with renal calcification.

Premature sick infants have been observed to develop diffuse nephrocalcinosis (noted in about two thirds of infants with birth weights of < 1500 g), typically when exposed to diuretic therapy or prolonged oxygen therapy. No clearly effective treatment has been established, [31]  although the natural history of this phenomenon is not well understood. [32]  Most cases resolve within a year [33, 34] and only a small proportion (< 15%) of stones require interventions. [34]  Urinary tract infections after birth represent another risk factor for slow resolution of these calcifications.

The presence of microscopic calcification in “generic” chronic kidney disease (CKD) is relatively understudied. One study documented progressive worsening of microscopic nephrocalcinosis with worsening stages of CKD (4.6, 14.3, 20.2, and 54.0% in patients with CKD stages 1-2,  3–4, and 5, respectively).  Lower serum bicarbonate level and higher serum PTH and calcium levels reresented indedendent risk factors for severity. [35]

There has been a growing awareness of the diffusely increased calcifications in patients with advanced renal failure and end-stage renal disease. [36] In the uremic environment, the use of large, pharmacologic doses of vitamin-D analogues and calcium-based phosphorus binders appears to accelerate the process. The presence of extraskeletal calcifications seems to be more closely correlated with the calcium-phosphorus product (sometimes referred to as the double product) and total-body calcium overload than with the presence of hypercalcemia. Calcifications in such cases are not limited to the kidneys but may involve multiple organs, including the heart, vascular beds, parenchymal organs, skin, and subcutaneous tissues.

 

The prognosis depends mainly on the etiology of the nephrocalcinosis. Patients with idiopathic hypercalciuria and medullary sponge kidney have the least risk of renal failure and the best prognosis, whereas patients with primary type 1 hyperoxaluria have the worst prognosis. [37]

The morbidity and mortality associated with nephrocalcinosis depend on the disease associated with the condition rather than on the nephrocalcinosis itself. [38]  The major long-term complication in patients with medullary nephrocalcinosis is renal failure. Early treatment of reversible causes of renal failure, such as urinary infections, obstruction, and hypertension, is essential. Once chronic renal failure has developed, treatment should focus on appropriate management of chronic kidney disease and its complications.

In educating patients about nephrocalcinosis, key points to emphasize include the following:

Nephrocalcinosis is usually an incidental finding

Hypercalcemia or hypercalciuria are frequently present

Nephrocalcinosis is more likely to be a consequence of an underlying abnormality than it is to be the cause

The exact epidemiology and disease burden for nephrocalcinosis is not known. Prematurity-associated calcifications are known to resolve, albeit with significant inter-individual variability. [33]  Younger age in general is very suspicious for underlying genetic disorders, such as Bartter syndrome, [39, 40]  Dent disease or variants of Dent disease, [41]  or amelogenesis imperfecta. [29, 30] Whole-exome sequencing is a novel and very powerful technology to detect a monogenic cause of nephrocalcinosis and early-onset nephrolithiasis.87 In older age groups, acquired processes such as distal renal tubular acidosis are more likely to occur. [10, 42]

Risk factors for nephrocalcinosis include the following:

 

 

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Tibor Fulop, MD, PhD, FACP, FASN Professor of Medicine, Department of Medicine, Division of Nephrology, Medical University of South Carolina College of Medicine; Attending Physician; Medical Services, Ralph H Johnson VA Medical Center

Tibor Fulop, MD, PhD, FACP, FASN is a member of the following medical societies: American Academy of Urgent Care Medicine, American College of Physicians, American Society of Diagnostic and Interventional Nephrology, American Society of Hypertension, American Society of Nephrology, International Society for Apheresis, International Society for Hemodialysis, Magyar Orvosi Kamara (Hungarian Chamber of Medicine), Southern Society for Clinical Investigation

Disclosure: Serve(d) as a director, officer, partner, employee, advisor, consultant or trustee for: Fresenius Medical Care, Hungary; Dialysis Clinic Inc., USA.

Sohail Abdul Salim, MD, FASN, FACP Consultant Physician, Central Nephrology; Affiliate Faculty, Department of Internal Medicine, Division of Nephrology, University of Mississippi Medical Center

Sohail Abdul Salim, MD, FASN, FACP is a member of the following medical societies: American College of Physicians, American Society of Nephrology, Mississippi State Medical Association, Renal Physicians Association

Disclosure: Nothing to disclose.

Vecihi Batuman, MD, FASN Huberwald Professor of Medicine, Section of Nephrology-Hypertension, Tulane University School of Medicine; Chief, Renal Section, Southeast Louisiana Veterans Health Care System

Vecihi Batuman, MD, FASN is a member of the following medical societies: American College of Physicians, American Society of Hypertension, American Society of Nephrology, International Society of Nephrology, Southern Society for Clinical Investigation

Disclosure: Nothing to disclose.

Mahendra Agraharkar, MD, MBBS, FACP, FASN Clinical Associate Professor of Medicine, Baylor College of Medicine; President and CEO, Space City Associates of Nephrology

Mahendra Agraharkar, MD, MBBS, FACP, FASN is a member of the following medical societies: American College of Physicians, American Society of Nephrology, National Kidney Foundation

Disclosure: Nothing to disclose.

Rajiv Gupta, MD Assistant Professor, Department of Medicine, Texas A&M Health Science Center College of Medicine; Consulting Staff, Veterans Affairs Medical Center

Rajiv Gupta, MD is a member of the following medical societies: Alpha Omega Alpha, American College of Cardiology, Society for Cardiovascular Angiography and Interventions

Disclosure: Nothing to disclose.

Eleanor Lederer, MD Professor of Medicine, Chief, Nephrology Division, Director, Nephrology Training Program, Director, Metabolic Stone Clinic, Kidney Disease Program, University of Louisville School of Medicine; Consulting Staff, Louisville Veterans Affairs Hospital

Eleanor Lederer, MD is a member of the following medical societies: American Association for the Advancement of Science, American Federation for Medical Research, American Society for Biochemistry and Molecular Biology, American Society for Bone and Mineral Research, American Society of Nephrology, American Society of Transplantation, International Society of Nephrology, Kentucky Medical Association, National Kidney Foundation, and Phi Beta Kappa

Disclosure: Dept of Veterans Affairs Grant/research funds Research

James W Lohr, MD Professor, Department of Internal Medicine, Division of Nephrology, Fellowship Program Director, University of Buffalo State University of New York School of Medicine and Biomedical Sciences

James W Lohr, MD is a member of the following medical societies: American College of Physicians, American Heart Association, American Society of Nephrology, and Central Society for Clinical Research

Disclosure: Alexion Salary Employment

Rupert Patel, MD Physician, Division of Nephrology, Houston, Texas

Disclosure: Nothing to disclose.

Francisco Talavera, PharmD, PhD Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Medscape Salary Employment

Acknowledgments

The primary author would like to thank Dr Gurvinder Suri, Renal Fellow in the Nephrology Division at the University of Mississippi Medical Center, for his valuable peer review.

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